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Western blot All lanes: DR1 antibody at 2.32ug/ml Lane 1: Rat gonad tissue Lane 2: PC-3 whole cell lysate Lane 3: Hela whole cell lysate Lane 4: 293T whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 19 kDa Observed band size: 23, 26 kDa
Western blot All lanes: DR1 antibody at 2.32ug/ml Lane 1: Rat gonad tissue Lane 2: PC-3 whole cell lysate Lane 3: Hela whole cell lysate Lane 4: 293T whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 19 kDa Observed band size: 23, 26 kDa
Western blot All lanes: DR1 antibody at 2.32ug/ml Lane 1: Rat gonad tissue Lane 2: PC-3 whole cell lysate Lane 3: Hela whole cell lysate Lane 4: 293T whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 19 kDa Observed band size: 23, 26 kDa

DR1 Antibody

CSB-PA007175ESR2HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetDR1
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Overview

  • Supplier
    Cusabio
  • Product Name
    DR1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1810
  • Target name
    DR1
  • Target description
    down-regulator of transcription 1
  • Target synonyms
    NC2; NC2B; NC2-BETA; NCB2; negative cofactor 2; negative cofactor 2-beta; protein Dr1; TATA-binding protein-associated phosphoprotein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ01658
  • Protein Name
    Protein Dr1
  • Scientific Description
    The association of the DR1/DRAP1 heterodimer with TBP results in a functional repression of both activated and basal transcription of class II genes. This interaction precludes the formation of a transcription-competent complex by inhibiting the association of TFIIA and/or TFIIB with TBP. Can bind to DNA on its own. Component of the ATAC complex, a complex with histone acetyltransferase activity on histones H3 and H4.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of DR1 using anti-DR1 antibody (A02204-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Sw620 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human A375 whole cell lysates, Lane 4: rat testis tissue lysates, Lane 5: mouse testis tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DR1 antigen affinity purified polyclonal antibody (Catalog # A02204-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DR1 at approximately 19KD. The expected band size for DR1 is at 19KD.
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