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Drebrin antibody

GTX111649
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetDBN1
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Overview

  • Supplier
    GeneTex
  • Product Name
    Drebrin antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:20000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID1627
  • Target name
    DBN1
  • Target description
    drebrin 1
  • Target synonyms
    D0S117E; developmentally-regulated brain protein; drebrin; drebrin A; drebrin E; drebrin E2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ16643
  • Protein Name
    Drebrin
  • Scientific Description
    The protein encoded by this gene is a cytoplasmic actin-binding protein thought to play a role in the process of neuronal growth. It is a member of the drebrin family of proteins that are developmentally regulated in the brain. A decrease in the amount of this protein in the brain has been implicated as a possible contributing factor in the pathogenesis of memory disturbance in Alzheimers disease. At least two alternative splice variants encoding different protein isoforms have been described for this gene. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Drebrin/DBN1 using anti-Drebrin/DBN1 antibody (A05530-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: monkey COS-7 whole cell lysates, Lane 5: rat brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Drebrin/DBN1 antigen affinity purified polyclonal antibody (Catalog # A05530-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Drebrin/DBN1 at approximately 120 kDa. The expected band size for Drebrin/DBN1 is at 120 kDa.
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