Duck MT (Melatonin) ELISA Kit

Assay Genie

Duck MT (Melatonin) ELISA Kit

12

ELISA

15.63-1000 pg/mL

4.92 pg/mL

Research Use Only

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Duck MT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Duck MT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Duck MT in the samples is then determined by comparing the OD of the samples to the standard curve.

2°C to 8°C

41116100