The data was published in the journal Front Oncol in 2019. PMID: 31024851
![FACS analysis of C6 cells using GTX80684 Dynein antibody [74.1] compared to an isotype control (blue). Dilution : 1 ug/test for 60 min at room temperature Fixation : 2% paraformaldehyde Permeabilization : 90% methanol and incubated for 10 minutes at room temperature](https://www.genetex.com/upload/website/prouct_img/normal/GTX80684/GTX80684_217_FACS_w_23061322_396.webp "FACS analysis of C6 cells using GTX80684 Dynein antibody [74.1] compared to an isotype control (blue). Dilution : 1 ug/test for 60 min at room temperature Fixation : 2% paraformaldehyde Permeabilization : 90% methanol and incubated for 10 minutes at room temperature")
![FACS analysis of Hela cells using GTX80684 Dynein antibody [74.1] compared to an isotype control (blue). Dilution : 2 ug/test for 60 min at room temperature Fixation : 2% paraformaldehyde Permeabilization : 90% methanol and incubated for 10 minutes at room temperature](https://www.genetex.com/upload/website/prouct_img/normal/GTX80684/GTX80684_218_FACS_w_23061322_682.webp "FACS analysis of Hela cells using GTX80684 Dynein antibody [74.1] compared to an isotype control (blue). Dilution : 2 ug/test for 60 min at room temperature Fixation : 2% paraformaldehyde Permeabilization : 90% methanol and incubated for 10 minutes at room temperature")
![FACS analysis of U87-MG cells using GTX80684 Dynein antibody [74.1] compared to an isotype control (blue). Dilution : 2 ug/test for 60 min at room temperature Fixation : 2% paraformaldehyde Permeabilization : 90% methanol and incubated for 10 minutes at room temperature](https://www.genetex.com/upload/website/prouct_img/normal/GTX80684/GTX80684_219_FACS_w_23061322_431.webp "FACS analysis of U87-MG cells using GTX80684 Dynein antibody [74.1] compared to an isotype control (blue). Dilution : 2 ug/test for 60 min at room temperature Fixation : 2% paraformaldehyde Permeabilization : 90% methanol and incubated for 10 minutes at room temperature")
![ICC/IF analysis of C6 cells using GTX80684 Dynein antibody [74.1]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:20 overnight at 4oC](https://www.genetex.com/upload/website/prouct_img/normal/GTX80684/GTX80684_892_ICC-IF_w_23061322_247.webp "ICC/IF analysis of C6 cells using GTX80684 Dynein antibody [74.1]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:20 overnight at 4oC")
![ICC/IF analysis of HeLa cells using GTX80684 Dynein antibody [74.1]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:20 overnight at 4oC](https://www.genetex.com/upload/website/prouct_img/normal/GTX80684/GTX80684_893_ICC-IF_w_23061322_525.webp "ICC/IF analysis of HeLa cells using GTX80684 Dynein antibody [74.1]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:20 overnight at 4oC")
![ICC/IF analysis of U-87 MG cells using GTX80684 Dynein antibody [74.1]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:20 overnight at 4oC](https://www.genetex.com/upload/website/prouct_img/normal/GTX80684/GTX80684_894_ICC-IF_w_23061322_978.webp "ICC/IF analysis of U-87 MG cells using GTX80684 Dynein antibody [74.1]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:20 overnight at 4oC")
The data was published in the journal Front Oncol in 2019. PMID: 31024851
Dynein antibody [74.1]
GTX80684
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityBovine, Human, Rat
TargetDYNLL1
Overview
- SupplierGeneTex
- Product NameDynein antibody [74.1]
- Delivery Days Customer9
- Application Supplier NoteWB: 1 microg/ml. ICC/IF: 2-4 microg/ml. FACS: 1-2 microg/test. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID74.1
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID404151
- Target nameDYNLL1
- Target descriptiondynein light chain LC8-type 1
- Target synonymsCDLC2, DNCL1, dynein light chain 1, cytoplasmic, cytoplasmic dynein light polypeptide
- HostMouse
- IsotypeIgG
- Protein IDP61285
- Protein NameDynein light chain 1, cytoplasmic
- Scientific DescriptionThe directed movement of membrane-bounded organelles is an important process for various cellular functions including membrane transport, secretion, and axonal transport. Cytoplasmic dynein, a ubiquitous minus end-directed microtubule-based motor protein, is believed to be responsible for the retrograde transport of membranous organelles from the synapse to the cell body in fast axonal transport. The cytoplasmic dynein complex is composed of two ~530 kDa heavy chains, and intermediate chains of ~74 kDa and ~53-59 kDa.
- ReactivityBovine, Human, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Huang FI, Wu YW, Sung TY, et al. MPT0G413, A Novel HDAC6-Selective Inhibitor, and Bortezomib Synergistically Exert Anti-tumor Activity in Multiple Myeloma Cells. Front Oncol. 2019,9:249. doi: 10.3389/fonc.2019.00249Read this paper