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ProductsBack/DYNLT1 / TCTEX-1 Antibody (aa1-50)

DYNLT1 / TCTEX-1 Antibody (aa1-50)

LS-C762338
LifeSpan BioSciences
ApplicationsImmunoPrecipitation, Western Blot
Product group Antibodies
TargetDYNLT1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    DYNLT1 / TCTEX-1 Antibody (aa1-50)
  • Delivery Days Customer
    23
  • Application Supplier Note
    Immunoprecipitation Range: 2-10 microg/mg lysate
  • Applications
    ImmunoPrecipitation, Western Blot
  • Applications Supplier
    IP, WB (1:10000 - 1:25000) Immunoprecipitation Range: 2-10 µg/mg lysate
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID6993
  • Target name
    DYNLT1
  • Target description
    dynein light chain Tctex-type 1
  • Target synonyms
    CW-1, TCTEL1, TCTEX1, tctex-1, dynein light chain Tctex-type 1, T-complex testis-specific protein 1 homolog, t-complex-associated-testis-expressed 1-like 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of DYNLT1 using anti-DYNLT1 antibody (A05556). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SW620 whole cell lysates, Lane 2: monkey COS-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DYNLT1 antigen affinity purified polyclonal antibody (Catalog # A05556) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DYNLT1 at approximately 15 kDa. The expected band size for DYNLT1 is at 12 kDa.
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