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Immunohistochemistry of paraffin-embedded human skeletal muscle tissue using CSB-PA007314LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human skeletal muscle tissue using CSB-PA007314LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human skeletal muscle tissue using CSB-PA007314LA01HU at dilution of 1:100

DYSF Antibody

CSB-PA007314LA01HU
Cusabio
ApplicationsImmunoFluorescence, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetDYSF
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Overview

  • Supplier
    Cusabio
  • Product Name
    DYSF Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8291
  • Target name
    DYSF
  • Target description
    dysferlin
  • Target synonyms
    dysferlin; dystrophy-associated fer-1-like 1; FER1L1; fer-1-like family member 1; fer-1-like protein 1; LGMD2B; LGMDR2; limb girdle muscular dystrophy 2B (autosomal recessive); MMD1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO75923
  • Protein Name
    Dysferlin
  • Scientific Description
    Key calcium ion sensor involved in the Ca(2+)-triggered synaptic vesicle-plasma membrane fusion. Plays a role in the sarcolemma repair mechanism of both skeletal muscle and cardiomyocytes that permits rapid resealing of membranes disrupted by mechanical stress (By similarity).
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Dysferlin/DYSF using anti-Dysferlin/DYSF antibody (A01234-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: rat heart tissue lysates, Lane 3: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Dysferlin/DYSF antigen affinity purified polyclonal antibody (Catalog # A01234-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Dysferlin/DYSF at approximately 280 kDa. The expected band size for Dysferlin/DYSF is at 237 kDa.
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