ICC/IF analysis of MDCK cells using GTX11512 E-Cadherin antibody [DECMA-1] at 1:3200. Cells were fixed and permeabilized with methanol followed by acetone.
![ICC/IF analysis of MDCK cells using GTX11512 E-Cadherin antibody [DECMA-1] at 1:3,200. Cells were fixed and permeabilized with cold methanol followed by acetone.](https://www.genetex.com/upload/website/prouct_img/normal/GTX11512/GTX11512_20170605_ICCIF_w_23060519_834.webp "ICC/IF analysis of MDCK cells using GTX11512 E-Cadherin antibody [DECMA-1] at 1:3,200. Cells were fixed and permeabilized with cold methanol followed by acetone.")
![WB analysis of MDCK cells using GTX11512 E-Cadherin antibody [DECMA-1] at 1:250.](https://www.genetex.com/upload/website/prouct_img/normal/GTX11512/GTX11512_20170605_WB_w_23060519_640.webp "WB analysis of MDCK cells using GTX11512 E-Cadherin antibody [DECMA-1] at 1:250.")
ICC/IF analysis of MDCK cells using GTX11512 E-Cadherin antibody [DECMA-1] at 1:3200. Cells were fixed and permeabilized with methanol followed by acetone.
E-Cadherin antibody [DECMA-1]
GTX11512
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Canine, Human, Mouse
TargetCdh1
Overview
- SupplierGeneTex
- Product NameE-Cadherin antibody [DECMA-1]
- Delivery Days Customer9
- Antibody SpecificityMonoclonal Anti-Uvomorulin/E-Cadherin was selected against the mouse cell adhesion molecule uvomorulin/E-Cadherin. The antibody localizes the cell surface glycoprotein uvomorulin/E-cadherin that has been found to be identical to L-CAM, Cell CAM 80/120, an
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDDECMA-1
- ConjugateUnconjugated
- Gene ID12550
- Target nameCdh1
- Target descriptioncadherin 1
- Target synonymsAA960649; ARC-1; cadherin-1; Eca; E-ca; Ecad; E-cad; E-cadh; E-cadherin; epithelial cadherin; L-C; L-CAM; Um; UVO; uvomorulin
- HostRat
- IsotypeIgG1
- Scientific DescriptionCell-cell interactions during embryonic development and in tissue organization involve specific cell-adhesion molecules (CAMs) that are functionally defined by antibodies that interfere with cell-cell adhesion. CAMs are expressed on early embryonic cells and persist in derivatives of all three germ layers. The best characterized CAMs are N-CAM (neural CAM) and L-CAM (liver CAM). The protein Uvomorulin, initially identified in embryonal carcinoma, is identical to L-CAM, E-Cadherin, Cell CAM 80/120 and ARC-1, each of which have been characterized in different systems. E-Cadherin has been characterized as a 120 kDa cell surface glycoprotein from which an 84 kDa fragment can be released by trypsin digestion in the presence of Ca2+.
- ReactivityBovine, Canine, Human, Mouse
- Storage Instruction2°C to 8°C,-20°C or -80°C
- UNSPSC12352203
References
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- Renal protective effects of empagliflozin via inhibition of EMT and aberrant glycolysis in proximal tubules. Li J et al., 2020 Mar 26, JCI InsightRead more
- Deficiency in Dipeptidyl Peptidase-4 Promotes Chemoresistance through the CXCL12/CXCR4/mTOR/TGFbeta Signaling Pathway in Breast Cancer Cells. Li S et al., 2020 Jan 26, Int J Mol SciRead more
- Non-canonical Wnt signalling regulates scarring in biliary disease via the planar cell polarity receptors. Wilson DH et al., 2020 Jan 23, Nat CommunRead more
- Dipeptidyl peptidase-4 plays a pathogenic role in BSA-induced kidney injury in diabetic mice. Takagaki Y et al., 2019 May 17, Sci RepRead more
- E-cadherin and LGN align epithelial cell divisions with tissue tension independently of cell shape. Hart KC et al., 2017 Jul 18, Proc Natl Acad Sci U S ARead more
- Cell division orientation is coupled to cell-cell adhesion by the E-cadherin/LGN complex. Gloerich M et al., 2017 Jan 3, Nat CommunRead more
- Vimentin coordinates fibroblast proliferation and keratinocyte differentiation in wound healing via TGF-beta-Slug signaling. Cheng F et al., 2016 Jul 26, Proc Natl Acad Sci U S ARead more
- Contribution of acidic melanoma cells undergoing epithelial-to-mesenchymal transition to aggressiveness of non-acidic melanoma cells. Peppicelli S et al., 2014 Apr, Clin Exp MetastasisRead more