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ProductsBack/E48 / LY6D Antibody

E48 / LY6D Antibody

LS-C395672
LifeSpan BioSciences
ApplicationsELISA
Product group Antibodies
TargetLY6D
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    E48 / LY6D Antibody
  • Delivery Days Customer
    23
  • Applications
    ELISA
  • Applications Supplier
    ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1.5 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID8581
  • Target name
    LY6D
  • Target description
    lymphocyte antigen 6 family member D
  • Target synonyms
    E48, Ly-6D, lymphocyte antigen 6D, e48 antigen, lymphocyte antigen 6 complex, locus D
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C,-80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of LY6D using anti-LY6D antibody (A09680). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LY6D antigen affinity purified polyclonal antibody (Catalog # A09680) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LY6D at approximately 16 kDa. The expected band size for LY6D is at 13 kDa.
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