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EAPP Antibody

PACO26369
Assay Genie
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetEAPP
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Overview

  • Supplier
    Assay Genie
  • Product Name
    EAPP Antibody
  • Delivery Days Customer
    9
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA, WB, IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID55837
  • Target name
    EAPP
  • Target description
    E2F associated phosphoprotein
  • Target synonyms
    BM036, C14orf11, E2F-associated phosphoprotein
  • Host
    Rabbit
  • Protein IDQ56P03
  • Protein Name
    E2F-associated phosphoprotein
  • Shelf life instruction
    12 months
  • Source
    Rabbit
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

Datasheet

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MSDS

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FAQ

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Figure 1. Western blot analysis of EAPP using anti-EAPP antibody (A07290-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human Hela whole cell lysates, Lane 3: human COLO-320 whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: human HEK293 whole cell lysates, Lane 6: human K562 whole cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EAPP antigen affinity purified polyclonal antibody (Catalog # A07290-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EAPP at approximately 45KD. The expected band size for EAPP is at 45KD.
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