Comparison of western blot analysis after immunoprecipitation with traditional secondary antibody (1) and EasyBlot anti-Rabbit IgG kit (GTX225856-01)(2). (A) Input, 20ug of HCT116 lysate. (B) and (C): HCT116 lysates were immunoprecipitated using 2.5 ug of normal rabbit IgG (B) or anti-p53 antibody (GTX102965)(C).? The precipitated protein was detected by GTX102965 diluted at 1:10000.
Comparison of western blot analysis after immunoprecipitation with traditional secondary antibody (1) and EasyBlot anti-Rabbit IgG kit (GTX225856-01)(2). (A) Input, 20ug of HCT116 lysate. (B) and (C): HCT116 lysates were immunoprecipitated using 2.5 ug of normal rabbit IgG (B) or anti-p53 antibody (GTX102965)(C).? The precipitated protein was detected by GTX102965 diluted at 1:10000.
EasyBlot anti-Rabbit IgG Kit (Optimized for Prot A/G)
GTX225856-01
ApplicationsWestern Blot
Product group Labware / Accesories
CategoryTechnique Specific Products
Overview
- SupplierGeneTex
- Product NameEasyBlot anti-Rabbit IgG Kit (Optimized for Prot A/G)
- Delivery Days Customer9
- Application Supplier NoteSuggested starting dilutions for WB is 1:1000. Use 2-5 microg of the IP antibody; do not exceed 5 microg. Completely reduce all IP samples by 100mM DTT (fresh prepared). For further detail, please refer to the protocol.Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
- ApplicationsWestern Blot
- CertificationResearch Use Only
- Concentration0.318 mg/ml
- ConjugateHRP
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41121816