Bio-Connect

EasyStep Human EGFR2(Epidermal Growth Factor Receptor 2) ELISA Kit

ORB2302957
Biorbyt
ReactivityHuman
Product group Assays
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Overview

  • Supplier
    Biorbyt
  • Product Name
    EasyStep Human EGFR2(Epidermal Growth Factor Receptor 2) ELISA Kit
  • Delivery Days Customer
    10
  • Application Supplier Note
    standard: 5000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human EGFR2, and the Human EGFR2 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human EGFR2. After TMB substrate solution is added, only those wells that contain Human EGFR2 and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Human EGFR2 in the samples is then determined by comparing the OD of the samples to the standard curve
  • Assay Detection Range
    78.13-5000 pg/mL
  • Assay Sensitivity
    16.9 pg/mL
  • Certification
    Research Use Only
  • Scientific Description
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human EGFR2, and the Human EGFR2 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human EGFR2. After TMB substrate solution is added, only those wells that contain Human EGFR2 and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Human EGFR2 in the samples is then determined by comparing the OD of the samples to the standard curve.
  • Reactivity
    Human
  • UNSPSC
    41116158