EasyStep Human hs-CRP(high sensitivity C Reactive Protein) ELISA Kit
ORB1950023
ReactivityHuman
Product group Assays
Overview
- SupplierBiorbyt
- Product NameEasyStep Human hs-CRP(high sensitivity C Reactive Protein) ELISA Kit
- Delivery Days Customer10
- Application Supplier Notestandard: 5000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CRP, and the Human CRP standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human CRP. After TMB substrate solution is added, only those wells that contain Human CRP and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Human CRP in the samples is then determined by comparing the OD of the samples to the standard curve
- Assay Detection Range78.13-5000 pg/mL
- Assay Sensitivity21 pg/mL
- CertificationResearch Use Only
- Scientific DescriptionThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CRP, and the Human CRP standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human CRP. After TMB substrate solution is added, only those wells that contain Human CRP and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Human CRP in the samples is then determined by comparing the OD of the samples to the standard curve.
- ReactivityHuman
- UNSPSC41116158


