EasyStep Human PSA(ProstateSpecific Antigen) ELISA Kit
ORB1817398
ReactivityHuman
Product group Assays
Overview
- SupplierBiorbyt
- Product NameEasyStep Human PSA(ProstateSpecific Antigen) ELISA Kit
- Delivery Days Customer10
- Application Supplier Notestandard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PSA, and the Human PSA standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human PSA. After TMB substrate solution is added, only those wells that contain Human PSA and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Human PSA in the samples is then determined by comparing the OD of the samples to the standard curve
- Assay Detection Range0.32-20 ng/mL
- Assay Sensitivity80 pg/mL
- CertificationResearch Use Only
- Scientific DescriptionThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PSA, and the Human PSA standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human PSA. After TMB substrate solution is added, only those wells that contain Human PSA and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Human PSA in the samples is then determined by comparing the OD of the samples to the standard curve.
- ReactivityHuman
- UNSPSC41116158


