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ECHS1 Polyclonal Antibody

BS-2920R
Bioss Antibodies
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetECHS1
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    ECHS1 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID1892
  • Target name
    ECHS1
  • Target description
    enoyl-CoA hydratase, short chain 1
  • Target synonyms
    ECHS1D; enoyl Coenzyme A hydratase, short chain, 1, mitochondrial; enoyl-CoA hydratase, mitochondrial; enoyl-CoA hydratase, short chain, 1, mitochondrial; epididymis secretory sperm binding protein; SCEH; short chain enoyl-CoA hydratase
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Immunohistochemical staining of human gastrointestinal, heart muscle, kidney and liver using Anti-ECHS1 antibody HPA021995 (A) shows similar protein distribution across tissues to independent antibody HPA022476 (B).
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Western blot All lanes: ECHS1 antibody at 3.03microg/ml Lane 1: Mouse liver tissue Lane 2: Mouse skeletal muscle tissue Lane 3: Hela whole cell lysate Lane 4: HepG2 whole cell lysate Lane 5: MCF-7 whole cell lysate Lane 6: Jurkat whole cell lysate Lane 7: A549 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 32 kDa Observed band size: 32 kDa
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Figure 1. Western blot analysis of ECHS1 using anti-ECHS1 antibody (A04508-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HT1080 whole cell lysates, Lane 4: human 293T whole cell lysates, Lane 5: human MCF-7 whole cell lysates, Lane 6: human U-87MG whole cell lysates, Lane 7: human CACO-2 whole cell lysates, Lane 8: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ECHS1 antigen affinity purified polyclonal antibody (Catalog # A04508-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ECHS1 at approximately 29 kDa. The expected band size for ECHS1 is at 29 kDa.
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