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Western Blot analysis of HepG2 cells using DCI Polyclonal Antibody
Western Blot analysis of HepG2 cells using DCI Polyclonal Antibody
Western Blot analysis of HepG2 cells using DCI Polyclonal Antibody

ECI1 Antibody

CSB-PA005174
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetECI1
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Overview

  • Supplier
    Cusabio
  • Product Name
    ECI1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1632
  • Target name
    ECI1
  • Target description
    enoyl-CoA delta isomerase 1
  • Target synonyms
    3,2 trans-enoyl-Coenzyme A isomerase; 3,2-trans-enoyl-CoA isomerase, mitochondrial; acetylene-allene isomerase; D3,D2-enoyl-CoA isomerase; DCI; delta(3),Delta(2)-enoyl-CoA isomerase; dodecenoyl-CoA delta isomerase (3,2 trans-enoyl-CoA isomerase); dodecenoyl-Coenzyme A delta isomerase (3,2 trans-enoyl-Coenzyme A isomerase); enoyl-CoA delta isomerase 1, mitochondrial; epididymis secretory sperm binding protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP42126
  • Protein Name
    Enoyl-CoA delta isomerase 1, mitochondrial
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of DCI using anti-DCI antibody (PA1763). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Rat Liver Tissue Lysate, Lane 2: Human Placenta Tissue Lysate, Lane 3: A549 Whole Cell Lysate, Lane 4: SMMC Whole Cell Lysate, Lane 5: COLO320 Whole Cell Lysate, Lane 6: HELA Whole Cell Lysate, Lane 7: HT1080 Whole Cell Lysate, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DCI antigen affinity purified polyclonal antibody (Catalog # PA1763) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DCI at approximately 33KD. The expected band size for DCI is at 33KD.
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