IHC image of CSB-PA888001LA01HU diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

ECT2 Antibody

CSB-PA888001LA01HU

ApplicationsImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry

Product group Antibodies

ReactivityHuman

TargetECT2

Overview

Supplier
Cusabio
Product Name
ECT2 Antibody
Delivery Days Customer
20
Applications
ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry
Certification
Research Use Only
Clonality
Polyclonal
Conjugate
Unconjugated
Gene ID1894
Target name
ECT2
Target description
epithelial cell transforming 2
Target synonyms
ARHGEF31, protein ECT2, epithelial cell-transforming sequence 2 oncogene
Host
Rabbit
Isotype
IgG
Protein IDQ9H8V3
Protein Name
Protein ECT2
Scientific Description
Guanine nucleotide exchange factor (GEF) that catalyzes the exchange of GDP for GTP. Promotes guanine nucleotide exchange on the Rho family members of small GTPases, like RHOA, RHOC, RAC1 and CDC42. Required for signal transduction pathways involved in the regulation of cytokinesis. Component of the centralspindlin complex that serves as a microtubule-dependent and Rho-mediated signaling required for the myosin contractile ring formation during the cell cycle cytokinesis. Regulates the translocation of RHOA from the central spindle to the equatorial region. Plays a role in the control of mitotic spindle assembly; regulates the activation of CDC42 in metaphase for the process of spindle fibers attachment to kinetochores before chromosome congression. Involved in the regulation of epithelial cell polarity; participates in the formation of epithelial tight junctions in a polarity complex PARD3-PARD6-protein kinase PRKCQ-dependent manner. Plays a role in the regulation of neurite outgrowth. Inhibits phenobarbital (PB)-induced NR1I3 nuclear translocation. Stimulates the activity of RAC1 through its association with the oncogenic PARD6A-PRKCI complex in cancer cells, thereby acting to coordinately drive tumor cell proliferation and invasion. Also stimulates genotoxic stress-induced RHOB activity in breast cancer cells leading to their cell death.
Reactivity
Human
Storage Instruction
-20°C or -80°C
UNSPSC
41116161

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ECT2 Antibody

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