Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with EDG8 Polyclonal Antibody, Unconjugated (bs-11457R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with EDG8 Polyclonal Antibody, Unconjugated (bs-11457R) at 1:200 overnight at 4°C, DAB staining.
EDG8 Polyclonal Antibody
BS-11457R
ApplicationsImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityEquine, Human, Mouse, Porcine, Rabbit, Rat, Sheep
TargetS1PR5
Overview
- SupplierBioss
- Product NameEDG8 Polyclonal Antibody
- Delivery Days Customer16
- ApplicationsImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- Applications SupplierELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 ug/ul
- ConjugateUnconjugated
- Gene ID53637
- Target nameS1PR5
- Target descriptionsphingosine-1-phosphate receptor 5
- Target synonymsEDG8, Edg-8, S1P5, SPPR-1, SPPR-2, sphingosine 1-phosphate receptor 5, S1P receptor 5, S1P receptor Edg-8, endothelial differentiation G-protein-coupled receptor 8, endothelial differentiation, sphingolipid G-protein-coupled receptor, 8, sphingosine 1-phosphate receptor EDG8, sphingosine 1-phosphate receptor Edg-8
- HostRabbit
- IsotypeIgG
- Protein IDQ9H228
- Protein NameSphingosine 1-phosphate receptor 5
- ReactivityEquine, Human, Mouse, Porcine, Rabbit, Rat, Sheep
- Storage Instruction-20°C
- UNSPSC12352203
References
- Xiao W, Fu S, Xu K, et al. Fingolimod Suppresses a Cascade of Core Vicious Cycle in Dry Eye NOD Mouse Model: Involvement of Sphingosine-1-Phosphate Receptors in Infiltrating Leukocytes. Invest Ophthalmol Vis Sci. 2017,58(14):6123-6132. doi: 10.1167/iovs.17-21445Read this paper
Datasheet
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