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Western Blot analysis of COS7 cells using ETAR Polyclonal Antibody
Western Blot analysis of COS7 cells using ETAR Polyclonal Antibody
Western Blot analysis of COS7 cells using ETAR Polyclonal Antibody

EDNRA Antibody

CSB-PA002447
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Monkey, Mouse
TargetEDNRA
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Overview

  • Supplier
    Cusabio
  • Product Name
    EDNRA Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1909
  • Target name
    EDNRA
  • Target description
    endothelin receptor type A
  • Target synonyms
    ET-A, ETA, ETA-R, ETAR, ETRA, MFDA, hET-AR, endothelin-1 receptor, G protein-coupled receptor, endothelin receptor subtype A, endothelin-1-specific receptor
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP25101
  • Protein Name
    Endothelin-1 receptor
  • Reactivity
    Human, Monkey, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Endothelin A Receptor/ET-A/ EDNRA using anti-Endothelin A Receptor/ET-A/ EDNRA antibody (A01828-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Endothelin A Receptor/ET-A/ EDNRA antigen affinity purified polyclonal antibody (Catalog # A01828-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Endothelin A Receptor/ET-A/ EDNRA at approximately 50KD. The expected band size for Endothelin A Receptor/ET-A/ EDNRA is at 50KD.
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