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ProductsBack/EDNRA / Endothelin A Receptor Antibody (C-Terminus, Biotin)

EDNRA / Endothelin A Receptor Antibody (C-Terminus, Biotin)

LS-C471611
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetEDNRA
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    EDNRA / Endothelin A Receptor Antibody (C-Terminus, Biotin)
  • Delivery Days Customer
    23
  • Application Supplier Note
    The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
  • Applications
    Western Blot
  • Applications Supplier
    WB The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Biotin
  • Estimated Purity
    ...
  • Gene ID1909
  • Target name
    EDNRA
  • Target description
    endothelin receptor type A
  • Target synonyms
    endothelin receptor subtype A; endothelin-1 receptor; endothelin-1-specific receptor; ETA; ET-A; ETAR; ETA-R; ETRA; G protein-coupled receptor; hET-AR; MFDA
  • Host
    Rabbit
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Endothelin A Receptor/ET-A/ EDNRA using anti-Endothelin A Receptor/ET-A/ EDNRA antibody (A01828-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Endothelin A Receptor/ET-A/ EDNRA antigen affinity purified polyclonal antibody (Catalog # A01828-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Endothelin A Receptor/ET-A/ EDNRA at approximately 50KD. The expected band size for Endothelin A Receptor/ET-A/ EDNRA is at 50KD.
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