EDTA buffer, 10X pH 8.0
GTX30937
Product group Chemicals
Overview
- SupplierGeneTex
- Product NameEDTA buffer, 10X pH 8.0
- Delivery Days Customer9
- Application Supplier NoteDeparafinize FFPE tissue section is carried out as outlined in your protocol. Hydrate tissue with PBS. The tissue slides are immersed in this 1X solution and heated for 10-20 minutes at 100C; OR in pressure cooker for 3-5 minutes followed by cooling at room temperature for about 20 minutes. The tissue sections are ready for further IHC protocol.
- CertificationResearch Use Only
- FormulationLiquid
- Scientific DescriptionThis buffer is intended for heat-induced antigen retriever on formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies. In IHC most commonly used fixative like formalin mask tissue antigens (cellular, membrane and nuclear) by cross-linking process, this results in poor or no staining in IHC. The use of this buffer on FFPE tissue section improves accessibility of antibodies to tissue antigens.
- Storage Instruction2°C to 8°C
- UNSPSC12161700