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EDTA buffer, 10X pH 8.0

Research Use Only
GTX30937
GeneTex
Product group Chemicals
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Overview

  • Supplier
    GeneTex
  • Product Name
    EDTA buffer, 10X pH 8.0
  • Delivery Days Customer
    9
  • Application Supplier Note
    Deparafinize FFPE tissue section is carried out as outlined in your protocol. Hydrate tissue with PBS. The tissue slides are immersed in this 1X solution and heated for 10-20 minutes at 100C; OR in pressure cooker for 3-5 minutes followed by cooling at room temperature for about 20 minutes. The tissue sections are ready for further IHC protocol.
  • Certification
    Research Use Only
  • Formulation
    Liquid
  • Scientific Description
    This buffer is intended for heat-induced antigen retriever on formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies. In IHC most commonly used fixative like formalin mask tissue antigens (cellular, membrane and nuclear) by cross-linking process, this results in poor or no staining in IHC. The use of this buffer on FFPE tissue section improves accessibility of antibodies to tissue antigens.
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12161700