
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using EEF1E1(GTX105658) antibody at 1:200 dilution.
EEF1E1 antibody [N1C3]
GTX105658
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetEEF1E1
Overview
- SupplierGeneTex
- Product NameEEF1E1 antibody [N1C3]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.2 mg/ml
- ConjugateUnconjugated
- Gene ID9521
- Target nameEEF1E1
- Target descriptioneukaryotic translation elongation factor 1 epsilon 1
- Target synonymsAIMP3, P18, eukaryotic translation elongation factor 1 epsilon-1, ARS-interacting multifunctional protein 3, aminoacyl tRNA synthetase complex-interacting multifunctional protein 3, multisynthase complex auxiliary component p18, multisynthetase complex auxiliary component p18, p18 component of aminoacyl-tRNA synthetase complex
- HostRabbit
- IsotypeIgG
- Protein IDO43324
- Protein NameEukaryotic translation elongation factor 1 epsilon-1
- Scientific DescriptionThis gene encodes a multifunctional protein that localizes both in the cytoplasm and in the nucleus. In the cytoplasm, the encoded protein is an auxiliary component of the macromolecular aminoacyl-tRNA synthase complex. However, its mouse homolog has been shown to translocate to the nucleus in response to DNA damage and plays a positive role in ATM/ATR-mediated p53 activation. Alternative splicing results in multiple transcript variants. [provided by RefSeq]
- ReactivityHuman, Mouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203

![Wild-type (WT) and EEF1E1 knockout (KO) 293T cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with EEF1E1 antibody [N1C3] (GTX105658) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Wild-type (WT) and EEF1E1 knockout (KO) 293T cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with EEF1E1 antibody [N1C3] (GTX105658) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX105658/GTX105658_39806_20180202_WB_KO_watermark_w_23060120_510.webp)
![EEF1E1 antibody [N1C3] detects EEF1E1 protein at cytoplasm on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. EEF1E1 antibody [N1C3] (GTX105658) diluted at 1:500.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min EEF1E1 antibody [N1C3] detects EEF1E1 protein at cytoplasm on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. EEF1E1 antibody [N1C3] (GTX105658) diluted at 1:500.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min](https://www.genetex.com/upload/website/prouct_img/normal/GTX105658/GTX105658_39806_20141205_IHC_M_2_w_23060120_279.webp)
![EEF1E1 antibody [N1C3] detects EEF1E1 protein at cytoplasm on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. EEF1E1 antibody [N1C3] (GTX105658) diluted at 1:500.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min EEF1E1 antibody [N1C3] detects EEF1E1 protein at cytoplasm on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. EEF1E1 antibody [N1C3] (GTX105658) diluted at 1:500.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min](https://www.genetex.com/upload/website/prouct_img/normal/GTX105658/GTX105658_39806_20141205_IHC_M_w_23060120_955.webp)






