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IHC-P analysis of formalin fixed human placenta tissue using GTX52497 EG-VEGF antibody [2L13].
IHC-P analysis of formalin fixed human placenta tissue using GTX52497 EG-VEGF antibody [2L13].
IHC-P analysis of formalin fixed human placenta tissue using GTX52497 EG-VEGF antibody [2L13].

EG-VEGF antibody [2L13]

GTX52497
GeneTex
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetPROK1
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Overview

  • Supplier
    GeneTex
  • Product Name
    EG-VEGF antibody [2L13]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:100 - 1:1000. IHC-P: 1:50 - 1:100. ELISA: 1:200 - 1:400. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    2L13
  • Concentration
    200 ug/ml
  • Conjugate
    Unconjugated
  • Gene ID84432
  • Target name
    PROK1
  • Target description
    prokineticin 1
  • Target synonyms
    black mamba toxin-related protein; EGVEGF; EG-VEGF; endocrine-gland-derived vascular endothelial growth factor; mambakine; PK1; PRK1; prokineticin-1
  • Host
    Mouse
  • Isotype
    IgG2
  • Protein IDP58294
  • Protein Name
    Prokineticin-1
  • Scientific Description
    The protein encoded by this gene induces proliferation, migration, and fenestration (the formation of membrane discontinuities) in capillary endothelial cells derived from endocrine glands. It has little or no effect on a variety of other endothelial and non-endothelial cell types. Its expression is restricted to the steroidogenic glands (ovary, testis, adrenal, and placenta), is induced by hypoxia, and often complementary to the expression of vascular endothelial growth factor (VEGF), suggesting that these molecules function in a coordinated manner. [provided by RefSeq, Sep 2011]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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IHC image of CSB-PA018746LA01HU diluted at 1:200 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Figure 1. Western blot analysis of Prokineticin 1 using anti-Prokineticin 1 antibody (PB9881). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Prokineticin 1 antigen affinity purified polyclonal antibody (Catalog # PB9881) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Prokineticin 1 at approximately 20 kDa. The expected band size for Prokineticin 1 is at 12 kDa.
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