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WB analysis of various samples using GTX66641 Eg5 antibody. Lane1 : Hela cell lysate Lane2 : sp2/0 cell lysate Lane3 : PC12 cell lysate Dilution : 1:500
WB analysis of various samples using GTX66641 Eg5 antibody. Lane1 : Hela cell lysate Lane2 : sp2/0 cell lysate Lane3 : PC12 cell lysate Dilution : 1:500
WB analysis of various samples using GTX66641 Eg5 antibody. Lane1 : Hela cell lysate Lane2 : sp2/0 cell lysate Lane3 : PC12 cell lysate Dilution : 1:500

Eg5 antibody

GTX66641
GeneTex
ApplicationsWestern Blot
DownloadDatasheet
Product group Antibodies
TargetKIF11
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Overview

  • Supplier
    GeneTex
  • Product Name
    Eg5 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID3832
  • Target name
    KIF11
  • Target description
    kinesin family member 11
  • Target synonyms
    EG5; HKSP; kinesin-like protein 1; kinesin-like protein KIF11; kinesin-like spindle protein HKSP; kinesin-related motor protein Eg5; KNSL1; MCLMR; thyroid receptor-interacting protein 5; TR-interacting protein 5; TRIP5; TRIP-5
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP52732
  • Protein Name
    Kinesin-like protein KIF11
  • Scientific Description
    This gene encodes a motor protein that belongs to the kinesin-like protein family. Members of this protein family are known to be involved in various kinds of spindle dynamics. The function of this gene product includes chromosome positioning, centrosome separation and establishing a bipolar spindle during cell mitosis. [provided by RefSeq, Jul 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Eg5/KIF11 using anti-Eg5/KIF11 antibody (A01754-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human Daudi whole cell lysates, Lane 5: human MOLT-4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Eg5/KIF11 antigen affinity purified polyclonal antibody (Catalog # A01754-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Eg5/KIF11 at approximately 130 kDa. The expected band size for Eg5/KIF11 is at 119 kDa.
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