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Western Blot analysis of 3T3 cells using EGF Polyclonal Antibody
Western Blot analysis of 3T3 cells using EGF Polyclonal Antibody
Western Blot analysis of 3T3 cells using EGF Polyclonal Antibody

EGF Antibody

CSB-PA007939
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetEGF
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Overview

  • Supplier
    Cusabio
  • Product Name
    EGF Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1950
  • Target name
    EGF
  • Target description
    epidermal growth factor
  • Target synonyms
    beta-urogastrone; HOMG4; pro-epidermal growth factor; URG
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP01133
  • Protein Name
    Pro-epidermal growth factor
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of EGF using anti-EGF antibody (PB9861). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: 22RV1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EGF antigen affinity purified polyclonal antibody (Catalog # PB9861) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EGF at approximately 134 kDa. The expected band size for EGF is at 133 kDa.
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