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Western Blot analysis of 3T3 cells using EGF Polyclonal Antibody
Western Blot analysis of 3T3 cells using EGF Polyclonal Antibody
Western Blot analysis of 3T3 cells using EGF Polyclonal Antibody

EGF Antibody

CSB-PA007939
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetEGF
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Overview

  • Supplier
    Cusabio
  • Product Name
    EGF Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1950
  • Target name
    EGF
  • Target description
    epidermal growth factor
  • Target synonyms
    HOMG4, URG, pro-epidermal growth factor, beta-urogastrone
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP01133
  • Protein Name
    Pro-epidermal growth factor
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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K562 cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with EGF Polyclonal Antibody(bs-2008R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
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Rat tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with pro-EGF antibody [N3C1], Internal (GTX111099) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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