EGLN3 Antibody

Cusabio

EGLN3 Antibody

20

ELISA, ImmunoHistoChemistry

Research Use Only

Polyclonal

Unconjugated

EGLN3

egl-9 family hypoxia inducible factor 3

HIFP4H3, HIFPH3, PHD3, prolyl hydroxylase EGLN3, HIF-PH3, HIF-prolyl hydroxylase 3, HPH-1, HPH-3, hypoxia-inducible factor prolyl hydroxylase 3, prolyl hydroxylase domain-containing protein 3

Rabbit

IgG

Prolyl hydroxylase EGLN3

Cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A. Also hydroxylates HIF2A. Has a preference for the CODD site for both HIF1A and HIF2A. Hydroxylation on the NODD site by EGLN3 appears to require prior hydroxylation on the CODD site. Hydroxylated HIFs are then targeted for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Under hypoxic conditions, the hydroxylation reaction is attenuated allowing HIFs to escape degradation resulting in their translocation to the nucleus, heterodimerization with HIF1B, and increased expression of hypoxy-inducible genes. EGLN3 is the most important isozyme in limiting physiological activation of HIFs (particularly HIF2A) in hypoxia. Also hydroxylates PKM in hypoxia, limiting glycolysis. Under normoxia, hydroxylates and regulates the stability of ADRB2. Regulator of cardiomyocyte and neuronal apoptosis. In cardiomyocytes, inhibits the anti-apoptotic effect of BCL2 by disrupting the BAX-BCL2 complex.

Human, Mouse, Rat

-20°C or -80°C

41116161