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Western Blot analysis of RAW264.7 and NIH/3T3 cell using EGR1 Polyclonal Antibody at dilution of 1:400
Western Blot analysis of RAW264.7 and NIH/3T3 cell using EGR1 Polyclonal Antibody at dilution of 1:400
Western Blot analysis of RAW264.7 and NIH/3T3 cell using EGR1 Polyclonal Antibody at dilution of 1:400

EGR1 Polyclonal Antibody

E-AB-10299
Elabscience
ApplicationsWestern Blot
Product group Antibodies
TargetEGR1
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Overview

  • Supplier
    Elabscience
  • Product Name
    EGR1 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    Western Blot
  • Applications Supplier
    ELISA WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID1958
  • Target name
    EGR1
  • Target description
    early growth response 1
  • Target synonyms
    AT225, G0S30, KROX-24, NGFI-A, TIS8, ZIF-268, ZIF268, ZNF225, early growth response protein 1, EGR-1, nerve growth factor-induced protein A, transcription factor ETR103, transcription factor Zif268, zinc finger gene 225, zinc finger protein 225, zinc finger protein Krox-24
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP18146
  • Protein Name
    Early growth response protein 1
  • Scientific Description
    EGR-1 (Early growth response protein 1) also known as Zif268 (zinc finger protein 225) or NGFI-A (nerve growth factor-induced protein A) is a protein that in humans is encoded by the EGR1 gene. The protein encoded by this gene belongs to the EGR family of C2H2-type zinc-finger proteins. It is a nuclear protein and functions as a transcriptional regulator. The products of target genes it activates are required for differentitation and mitogenesis. Studies suggest this is a cancer suppresor gene. Recognizes and binds to the DNA sequence 5-CGCCCCCGC-3(EGR-site).
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Egr1 using anti-Egr1 antibody (A00687-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human 22RV1 whole cell lysates, Lane 2: human PANC-1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Egr1 antigen affinity purified polyclonal antibody (Catalog # A00687-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Egr1 at approximately 75KD. The expected band size for Egr1 is at 58KD.
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