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Western blot analysis of extracts from K562 cells, using EGR2 antibody.
Western blot analysis of extracts from K562 cells, using EGR2 antibody.
Western blot analysis of extracts from K562 cells, using EGR2 antibody.

EGR2 Antibody

CSB-PA891042
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetEGR2
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Overview

  • Supplier
    Cusabio
  • Product Name
    EGR2 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1959
  • Target name
    EGR2
  • Target description
    early growth response 2
  • Target synonyms
    AT591, CMT1D, CMT4E, KROX20, E3 SUMO-protein ligase EGR2, E3 SUMO-protein transferase ERG2, KROX-20, Drosophila, homolog (early growth response-2), early growth response protein 2, zinc finger protein Krox-20
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP11161
  • Protein Name
    E3 SUMO-protein ligase EGR2
  • Scientific Description
    Sequence-specific DNA-binding transcription factor. Binds to two specific DNA sites located in the promoter region of HOXA4. E3 SUMO-protein ligase helping SUMO1 conjugation to its coregulators NAB1 and NAB2, whose sumoylation down-regulates EGR2 own transcriptional activity. Joseph L.J., Proc. Natl. Acad. Sci. U.S.A. 85:7164-7168(1988). Warner L.E., Nat. Genet. 18:382-384(1998). Bechtel S., BMC Genomics 8:399-399(2007).
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of EGR2 using anti-EGR2 antibody (A00921-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: human U20S whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat thymus tissue lysates, Lane 6: rat PC-12 whole cell lysates, Lane 7: mouse thymus tissue lysates, Lane 8: mouse Raw264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EGR2 antigen affinity purified polyclonal antibody (Catalog # A00921-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EGR2 at approximately 50 kDa. The expected band size for EGR2 is at 50,45 kDa.
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Lane 1: HepG2 cell lysates; Lane 2: MCF-7 cell lysates probed with EGR2 Polyclonal Antibody, Unconjugated (bs-8368R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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