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WB analysis of various sample lysates using GTX33172 EIF2B1 antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX33172 EIF2B1 antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX33172 EIF2B1 antibody. Dilution : 1:1000 Loading : 25microg per lane

EIF2B1 antibody

GTX33172
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
TargetEIF2B1
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Overview

  • Supplier
    GeneTex
  • Product Name
    EIF2B1 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500 - 1:2000. ICC/IF: 1:50 - 1:200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1967
  • Target name
    EIF2B1
  • Target description
    eukaryotic translation initiation factor 2B subunit alpha
  • Target synonyms
    EIF2B; eIF-2B GDP-GTP exchange factor subunit alpha; EIF2BA; eukaryotic translation initiation factor 2B, subunit 1 alpha, 26kDa; translation initiation factor eIF-2B subunit alpha
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ14232
  • Protein Name
    Translation initiation factor eIF-2B subunit alpha
  • Scientific Description
    This gene encodes one of five subunits of eukaryotic translation initiation factor 2B (EIF2B), a GTP exchange factor for eukaryotic initiation factor 2 and an essential regulator for protein synthesis. Mutations in this gene and the genes encoding other EIF2B subunits have been associated with leukoencephalopathy with vanishing white matter. [provided by RefSeq, Oct 2009]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of EIF2B1 using anti-EIF2B1 antibody (A04239-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: rat kidney tissue lysates, Lane 3: mouse liver tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EIF2B1 antigen affinity purified polyclonal antibody (Catalog # A04239-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EIF2B1 at approximately 34KD. The expected band size for EIF2B1 is at 34KD.
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