IHC-P analysis of human breast tissue using GTX54741 eIF2B3 antibody [1H3]. Right : Primary antibody Left : Negative control without primary antibody Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min Dilution : 1:200
![IHC-P analysis of human uterus tissue using GTX54741 eIF2B3 antibody [1H3]. Right : Primary antibody Left : Negative control without primary antibody Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min Dilution : 1:100](https://www.genetex.com/upload/website/prouct_img/normal/GTX54741/GTX54741_1368_IHC-P_w_23060900_925.webp "IHC-P analysis of human uterus tissue using GTX54741 eIF2B3 antibody [1H3]. Right : Primary antibody Left : Negative control without primary antibody Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min Dilution : 1:100")
![IP analysis of U2OS cell lysates using GTX54741 eIF2B3 antibody [1H3]. IP reaction : 3microg antibody / 500microg lysate Dilution : 1:1000](https://www.genetex.com/upload/website/prouct_img/normal/GTX54741/GTX54741_1456_IP_w_23060900_599.webp "IP analysis of U2OS cell lysates using GTX54741 eIF2B3 antibody [1H3]. IP reaction : 3microg antibody / 500microg lysate Dilution : 1:1000")
![WB analysis of 80microg whole cell lysate using GTX54741 eIF2B3 antibody [1H3]. Dilution : 1:1000](https://www.genetex.com/upload/website/prouct_img/normal/GTX54741/GTX54741_2128_WB_w_23060900_735.webp "WB analysis of 80microg whole cell lysate using GTX54741 eIF2B3 antibody [1H3]. Dilution : 1:1000")
![ICC/IF analysis of COS7 cells using GTX54741 eIF2B3 antibody [1H3]. Cells were probed without (left) or with(right) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute Dilution : 1:50 and incubated overnight](https://www.genetex.com/upload/website/prouct_img/normal/GTX54741/GTX54741_794_ICC-IF_w_23060900_206.webp "ICC/IF analysis of COS7 cells using GTX54741 eIF2B3 antibody [1H3]. Cells were probed without (left) or with(right) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute Dilution : 1:50 and incubated overnight")
![ICC/IF analysis of HeLa cells using GTX54741 eIF2B3 antibody [1H3]. Cells were probed without (left) or with(right) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute Dilution : 1:50 and incubated overnight](https://www.genetex.com/upload/website/prouct_img/normal/GTX54741/GTX54741_795_ICC-IF_w_23060900_122.webp "ICC/IF analysis of HeLa cells using GTX54741 eIF2B3 antibody [1H3]. Cells were probed without (left) or with(right) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute Dilution : 1:50 and incubated overnight")
IHC-P analysis of human breast tissue using GTX54741 eIF2B3 antibody [1H3]. Right : Primary antibody Left : Negative control without primary antibody Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min Dilution : 1:200
eIF2B3 antibody [1H3]
GTX54741
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Primate, Rat
TargetEIF2B3
Overview
- SupplierGeneTex
- Product NameeIF2B3 antibody [1H3]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:1000. ICC/IF: 1:10-1:100. IHC-P: 1:100-1:200. IP: 3 microg. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID1H3
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID8891
- Target nameEIF2B3
- Target descriptioneukaryotic translation initiation factor 2B subunit gamma
- Target synonymsEIF-2B; eIF-2B GDP-GTP exchange factor subunit gamma; EIF2Bgamma; eukaryotic translation initiation factor 2B, subunit 3 gamma, 58kDa; translation initiation factor eIF-2B subunit gamma
- HostMouse
- IsotypeIgG1
- Protein IDQ9NR50
- Protein NameTranslation initiation factor eIF-2B subunit gamma
- Scientific DescriptionThe protein encoded by this gene is one of the subunits of initiation factor eIF2B, which catalyzes the exchange of eukaryotic initiation factor 2-bound GDP for GTP. It has also been found to function as a cofactor of hepatitis C virus internal ribosome entry site-mediated translation. Mutations in this gene have been associated with leukodystrophy with vanishing white matter. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009]
- ReactivityHuman, Primate, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203