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Western Blot Positive WB detected in: MCF-7 whole cell lysate, Hela whole cell lysate, A375 whole cell lysate All lanes: EIF3C antibody at 3.4ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 106, 105 kDa Observed band size: 106 kDa
Western Blot Positive WB detected in: MCF-7 whole cell lysate, Hela whole cell lysate, A375 whole cell lysate All lanes: EIF3C antibody at 3.4ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 106, 105 kDa Observed band size: 106 kDa
Western Blot Positive WB detected in: MCF-7 whole cell lysate, Hela whole cell lysate, A375 whole cell lysate All lanes: EIF3C antibody at 3.4ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 106, 105 kDa Observed band size: 106 kDa

EIF3C Antibody

CSB-PA857867LA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetEIF3C
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Overview

  • Supplier
    Cusabio
  • Product Name
    EIF3C Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8663
  • Target name
    EIF3C
  • Target description
    eukaryotic translation initiation factor 3 subunit C
  • Target synonyms
    EIF3CL, EIF3S8, eIF3-p110, eukaryotic translation initiation factor 3 subunit C, cell migration-inducing protein 17, eukaryotic translation initiation factor 3 subunit 8, eukaryotic translation initiation factor 3, subunit 8 (110kD), eukaryotic translation initiation factor 3, subunit 8, 110kDa
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ99613
  • Protein Name
    Eukaryotic translation initiation factor 3 subunit C
  • Scientific Description
    Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of EIF3C using anti-EIF3C antibody (A06470-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat pancreas tissue lysates, Lane 2: mouse kidney tissue lysates, Lane 3: mouse pancreas tissue lysates, Lane 4: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EIF3C antigen affinity purified polyclonal antibody (Catalog # A06470-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EIF3C at approximately 105 kDa. The expected band size for EIF3C is at 105 kDa.
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