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WB analysis of various sample lysates using GTX32579 eIF3e antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX32579 eIF3e antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX32579 eIF3e antibody. Dilution : 1:1000 Loading : 25microg per lane

eIF3e antibody

GTX32579
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
TargetEIF3E
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Overview

  • Supplier
    GeneTex
  • Product Name
    eIF3e antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500 - 1:2000. ICC/IF: 1:50 - 1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3646
  • Target name
    EIF3E
  • Target description
    eukaryotic translation initiation factor 3 subunit E
  • Target synonyms
    EIF3-P48, EIF3S6, INT6, eIF3-p46, eukaryotic translation initiation factor 3 subunit E, eIF-3 p48, eukaryotic translation initiation factor 3 subunit 6, eukaryotic translation initiation factor 3, subunit 6 (48kD), eukaryotic translation initiation factor 3, subunit 6 48kDa, mammary tumor-associated protein INT6, murine mammary tumor integration site 6 (oncogene homolog), viral integration site protein INT-6 homolog
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP60228
  • Protein Name
    Eukaryotic translation initiation factor 3 subunit E
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

Datasheet

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Figure 1. Western blot analysis of EIF3e using anti-EIF3e antibody (A00481-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat thymus tissue lysates, Lane 2: mouse thymus tissue lysates, Lane 3: mouse spleen tissue lysates, Lane 4: COLO320 whole cell lysates, Lane 5: HELA whole Cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EIF3e antigen affinity purified polyclonal antibody (Catalog # A00481-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EIF3e at approximately 52KD. The expected band size for EIF3e is at 52KD.
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