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IP analysis of 293T cell lysate using GTX33175 eIF4B antibody. Antibody amount : 1microg / 200microg lysate Dilution : 1:1000
IP analysis of 293T cell lysate using GTX33175 eIF4B antibody. Antibody amount : 1microg / 200microg lysate Dilution : 1:1000
IP analysis of 293T cell lysate using GTX33175 eIF4B antibody. Antibody amount : 1microg / 200microg lysate Dilution : 1:1000

eIF4B antibody

GTX33175
GeneTex
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetEIF4B
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Overview

  • Supplier
    GeneTex
  • Product Name
    eIF4B antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500 - 1:2000. ICC/IF: 1:50 - 1:100. IHC-P: 1:50 - 1:200. IP: 1:50 - 1:200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1975
  • Target name
    EIF4B
  • Target description
    eukaryotic translation initiation factor 4B
  • Target synonyms
    EIF-4B; eukaryotic translation initiation factor 4B; PRO1843
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP23588
  • Protein Name
    Eukaryotic translation initiation factor 4B
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of EIF4B using anti-EIF4B antibody (A00846-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human PANC-1 whole cell lysates, Lane 4: human HL-60 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EIF4B antigen affinity purified polyclonal antibody (Catalog # A00846-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EIF4B at approximately 80 kDa. The expected band size for EIF4B is at 80 kDa.
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