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Western blot analysis of extracts of various cell lines using EIF5A Polyclonal Antibody at dilution of 1:1000.
Western blot analysis of extracts of various cell lines using EIF5A Polyclonal Antibody at dilution of 1:1000.
Western blot analysis of extracts of various cell lines using EIF5A Polyclonal Antibody at dilution of 1:1000.

EIF5A Polyclonal Antibody

E-AB-60556
Elabscience
Product group Antibodies

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Overview

  • Supplier
    Elabscience
  • Product Name
    EIF5A Polyclonal Antibody
  • Delivery Days Customer
    12
  • Certification
    Research Use Only
  • Scientific Description
    EIF5A (Eukaryotic Translation Initiation Factor 5A) is a Protein Coding gene. Diseases associated with EIF5A include Human Immunodeficiency Virus Type 1 and Retinitis Pigmentosa 14. Among its related pathways are eIF5A regulation in response to inhibition of the nuclear export system and Metabolism of proteins. Gene Ontology (GO) annotations related to this gene include protein N-terminus binding. An important paralog of this gene is EIF5AL1.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of EIF5A using anti-EIF5A antibody (A01727-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: rat kidney tissue lysates, Lane 4: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EIF5A antigen affinity purified polyclonal antibody (Catalog # A01727-3) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EIF5A at approximately 18 kDa. The expected band size for EIF5A is at 18 kDa.
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