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Elafin antibody

GTX129581
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetPI3
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Overview

  • Supplier
    GeneTex
  • Product Name
    Elafin antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID5266
  • Target name
    PI3
  • Target description
    peptidase inhibitor 3
  • Target synonyms
    ESI, SKALP, WAP3, WFDC14, cementoin, elafin, PI-3, WAP four-disulfide core domain 14, WAP four-disulfide core domain protein 14, elastase-specific inhibitor, peptidase inhibitor 3, skin-derived, pre-elafin, protease inhibitor 3, skin-derived (SKALP), protease inhibitor WAP3, skin-derived antileukoproteinase, trappin-2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP19957
  • Protein Name
    Elafin
  • Scientific Description
    Neutrophil and pancreatic elastase-specific inhibitor of skin. It may prevent elastase-mediated tissue proteolysis.
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

Datasheet

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Figure 1. Western blot analysis of Elafin/Skalp using anti-Elafin/Skalp antibody (PB9957). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Elafin/Skalp antigen affinity purified polyclonal antibody (Catalog # PB9957) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Elafin/Skalp at approximately 25 kDa. The expected band size for Elafin/Skalp is at 12 kDa.
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