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Western blot All lanes: ELF1 antibody at 1.88ug/ml + Hela whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 68, 65 kDa Observed band size: 68 kDa
Western blot All lanes: ELF1 antibody at 1.88ug/ml + Hela whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 68, 65 kDa Observed band size: 68 kDa
Western blot All lanes: ELF1 antibody at 1.88ug/ml + Hela whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 68, 65 kDa Observed band size: 68 kDa

ELF1 Antibody

CSB-PA007596DSR1HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetELF1
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Overview

  • Supplier
    Cusabio
  • Product Name
    ELF1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1997
  • Target name
    ELF1
  • Target description
    E74 like ETS transcription factor 1
  • Target synonyms
    E74-like factor 1 (ets domain transcription factor); EFTUD1; ETS-related transcription factor Elf-1; RIA1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP32519
  • Protein Name
    ETS-related transcription factor Elf-1
  • Scientific Description
    Transcription factor that activates the LYN and BLK promoters. Appears to be required for the T-cell-receptor-mediated trans activation of HIV-2 gene expression. Binds specifically to two purine-rich motifs in the HIV-2 enhancer.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of E74 like factor 1 using anti-E74 like factor 1 antibody (A03187-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human COLO-320 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human PANC-1 whole cell lysates, Lane 5: Human A431 whole cell lysates, Lane 6: human Jurkat whole cell lysates, Lane 7: human 293T whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-E74 like factor 1 antigen affinity purified polyclonal antibody (Catalog # A03187-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for E74 like factor 1 at approximately 75KD. The expected band size for E74 like factor 1 is at 67KD.
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