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ELISA Kit for Fibroblast Activation Protein Alpha (FAPa)

SEC469HU
Cloud-Clone Corp.
Product group Assays
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Overview

  • Supplier
    Cloud-Clone Corp.
  • Product Name
    ELISA Kit for Fibroblast Activation Protein Alpha (FAPa)
  • Delivery Days Customer
    12
  • Assay Detection Range
    0.78-50ng/mL
  • Assay Precision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fibroblast Activation Protein Alpha (FAPa) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with ...
  • Assay Sensitivity
    The minimum detectable dose of this kit is typically less than 0.29ng/mL
  • Assay Specificity
    This assay has high sensitivity and excellent specificity for detection of Fibroblast Activation Protein Alpha (FAPa). No significant cross-reactivity or interference between Fibroblast Activation Protein Alpha (FAPa) and analogues was observed.
  • Assay Test Principle
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fibroblast Activation Protein Alpha (FAPa). Standards or samples are then added to the ...
  • Assay Time
    3h
  • Certification
    Research Use Only
  • UNSPSC
    41116158

References

  • Increased Expression of Fibroblast Activation Protein is Associated with Autophagy Dysregulation and Oxidative Stress in Obese Women with Uterine Fibroids. Maghraby N et al., 2022 Feb, Reprod Sci
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Illustration of the assay principle. Fluorogenic DPP Substrate 1 is a fluorogenic peptide substrate (Ala-Pro-AMC dipeptide). In the conjugated form the energy emitted from the fluorochrome AMC is quenched. Proteolysis releases AMC and fluorescence is emitted. The increase in fluorescence is directly proportional to FAP activity.
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