_w_23053123_477.webp)
WB analysis of various samples using GTX02854 ELK1 antibody [GT1257]. Dilution : 1:1000 Loading : 25microg
![Various whole cell extracts (30 microg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ELK1 antibody [GT1257] (GTX02854) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX02854/GTX02854_4000000339_20210122_WB_2_w_23053123_625.webp "Various whole cell extracts (30 microg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ELK1 antibody [GT1257] (GTX02854) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![HCT116 whole cell and nuclear extracts (30 microg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ELK1 antibody [GT1257] (GTX02854) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX02854/GTX02854_4000000339_20210122_WB_Fraction_2_w_23053123_715.webp "HCT116 whole cell and nuclear extracts (30 microg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ELK1 antibody [GT1257] (GTX02854) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![293T whole cell and nuclear extracts (30 microg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ELK1 antibody [GT1257] (GTX02854) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX02854/GTX02854_4000000339_20210122_WB_Fraction_w_23053123_551.webp "293T whole cell and nuclear extracts (30 microg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ELK1 antibody [GT1257] (GTX02854) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Whole cell extract (30 microg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ELK1 antibody [GT1257] (GTX02854) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX02854/GTX02854_4000000339_20210122_WB_w_23053123_782.webp "Whole cell extract (30 microg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ELK1 antibody [GT1257] (GTX02854) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
WB analysis of various samples using GTX02854 ELK1 antibody [GT1257]. Dilution : 1:1000 Loading : 25microg
ELK1 antibody [GT1257]
GTX02854
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetELK1
Overview
- SupplierGeneTex
- Product NameELK1 antibody [GT1257] - Orthogonal Validated
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500 - 1:2000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDGT1257
- Concentration0.52 mg/ml
- ConjugateUnconjugated
- Gene ID2002
- Target nameELK1
- Target descriptionETS transcription factor ELK1
- Target synonymsELK1, ETS transcription factor; ELK1, member of ETS oncogene family; ETS domain-containing protein Elk-1; ETS-like gene 1; tyrosine kinase (ELK1) oncogene
- HostRabbit
- IsotypeIgG
- Protein IDP19419
- Protein NameETS domain-containing protein Elk-1
- Scientific DescriptionThis gene is a member of the Ets family of transcription factors and of the ternary complex factor (TCF) subfamily. Proteins of the TCF subfamily form a ternary complex by binding to the the serum response factor and the serum response element in the promoter of the c-fos proto-oncogene. The protein encoded by this gene is a nuclear target for the ras-raf-MAPK signaling cascade. This gene produces multiple isoforms by using alternative translational start codons and by alternative splicing. Related pseudogenes have been identified on chromosomes 7 and 14. [provided by RefSeq, Mar 2012]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203