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IHC-P analysis of formalin fixed human breast cancer tissue section using GTX56096 ELL antibody. Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)
IHC-P analysis of formalin fixed human breast cancer tissue section using GTX56096 ELL antibody. Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)
IHC-P analysis of formalin fixed human breast cancer tissue section using GTX56096 ELL antibody. Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)

ELL antibody

GTX56096
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetELL
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Overview

  • Supplier
    GeneTex
  • Product Name
    ELL antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500 - 1:1000. IHC-P: 1:100 - 1:200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8178
  • Target name
    ELL
  • Target description
    elongation factor for RNA polymerase II
  • Target synonyms
    C19orf17, ELL1, MEN, PPP1R68, RNA polymerase II elongation factor ELL, ELL gene (11-19 lysine-rich leukemia gene), ELL/KMT2A fusion protein, KMT2A/ELL fusion protein, eleven-nineteen lysine-rich leukemia protein, elongation factor RNA polymerase II, protein phosphatase 1, regulatory subunit 68
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP55199
  • Protein Name
    RNA polymerase II elongation factor ELL
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ELL using anti-ELL antibody (A03336-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human HL-60 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ELL antigen affinity purified polyclonal antibody (Catalog # A03336-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ELL at approximately 60-80 kDa. The expected band size for ELL is at 68 kDa.
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