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ProductsBack/EMD Antibody
Western Blot Positive WB detected in: Hela whole cell lysate, A549 whole cell lysate, Colo320 whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, MCF-7 whole cell lysate, Mouse lung tissue All lanes: EMD antibody at 2microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 29 kDa Observed band size: 35 kDa
Western Blot Positive WB detected in: Hela whole cell lysate, A549 whole cell lysate, Colo320 whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, MCF-7 whole cell lysate, Mouse lung tissue All lanes: EMD antibody at 2microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 29 kDa Observed band size: 35 kDa
Western Blot Positive WB detected in: Hela whole cell lysate, A549 whole cell lysate, Colo320 whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, MCF-7 whole cell lysate, Mouse lung tissue All lanes: EMD antibody at 2microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 29 kDa Observed band size: 35 kDa

EMD Antibody

CSB-PA007633LA01HU
Cusabio
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetEMD
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Overview

  • Supplier
    Cusabio
  • Product Name
    EMD Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2010
  • Target name
    EMD
  • Target description
    emerin
  • Target synonyms
    EDMD, LEMD5, STA, emerin, LEM domain containing 5
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP50402
  • Protein Name
    Emerin
  • Scientific Description
    Stabilizes and promotes the formation of a nuclear actin cortical network. Stimulates actin polymerization in vitro by binding and stabilizing the pointed end of growing filaments. Inhibits beta-catenin activity by preventing its accumulation in the nucleus. Acts by influencing the nuclear accumulation of beta-catenin through a CRM1-dependent export pathway. Links centrosomes to the nuclear envelope via a microtubule association. EMD and BAF are cooperative cofactors of HIV-1 infection. Association of EMD with the viral DNA requires the presence of BAF and viral integrase. The association of viral DNA with chromatin requires the presence of BAF and EMD. Required for proper localization of non-farnesylated prelamin-A/C.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 2. IHC analysis of Emerin using anti-Emerin antibody (A00714). Emerin was detected in paraffin-embedded section of human endometrial carcinoma tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2microg/ml rabbit anti-Emerin Antibody (A00714) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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