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Western Blot Positive WB detected in: A549 whole cell lysate, Hela whole cell lysate All lanes: EML4 antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 109, 103 kDa Observed band size: 109 kDa
Western Blot Positive WB detected in: A549 whole cell lysate, Hela whole cell lysate All lanes: EML4 antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 109, 103 kDa Observed band size: 109 kDa
Western Blot Positive WB detected in: A549 whole cell lysate, Hela whole cell lysate All lanes: EML4 antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 109, 103 kDa Observed band size: 109 kDa

EML4 Antibody

CSB-PA862066LA01HU
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
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Overview

  • Supplier
    Cusabio
  • Product Name
    EML4 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96HE3
  • Protein Name
    EML4 protein
  • Scientific Description
    May modify the assembly dynamics of microtubules, such that microtubules are slightly longer, but more dynamic.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of EML4 using anti-EML4 antibody (A00930-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates, Lane 3: mouse lung tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EML4 antigen affinity purified polyclonal antibody (Catalog # A00930-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EML4 at approximately 120KD. The expected band size for EML4 is at 109KD.
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