FACS analysis of 293T cells using GTX22786 Endothelin 1 antibody [TR.ET.48.5] compared to an isotype control (blue). Dilution : 0.5 ug/test for 60 min at room temperature Fixation : 2% paraformaldehyde
![FACS analysis of 3T3 cells using GTX22786 Endothelin 1 antibody [TR.ET.48.5] compared to an isotype control (blue). Dilution : 0.5 ug/test for 60 min at room temperature Fixation : 2% paraformaldehyde](https://www.genetex.com/upload/website/prouct_img/normal/GTX22786/GTX22786_142_FACS_w_23060620_738.webp "FACS analysis of 3T3 cells using GTX22786 Endothelin 1 antibody [TR.ET.48.5] compared to an isotype control (blue). Dilution : 0.5 ug/test for 60 min at room temperature Fixation : 2% paraformaldehyde")
![FACS analysis of HepG2 cells using GTX22786 Endothelin 1 antibody [TR.ET.48.5] compared to an isotype control (blue). Dilution : 0.5 ug/test for 60 min at room temperature Fixation : 2% paraformaldehyde](https://www.genetex.com/upload/website/prouct_img/normal/GTX22786/GTX22786_143_FACS_w_23060620_118.webp "FACS analysis of HepG2 cells using GTX22786 Endothelin 1 antibody [TR.ET.48.5] compared to an isotype control (blue). Dilution : 0.5 ug/test for 60 min at room temperature Fixation : 2% paraformaldehyde")
![WB analysis of 25 ug of PC12 cell lysate using GTX22786 Endothelin 1 antibody [TR.ET.48.5]. Dilution : 1:500](https://www.genetex.com/upload/website/prouct_img/normal/GTX22786/GTX22786_1592_WB_w_23060620_574.webp "WB analysis of 25 ug of PC12 cell lysate using GTX22786 Endothelin 1 antibody [TR.ET.48.5]. Dilution : 1:500")
![ICC/IF analysis of Hela cells using GTX22786 Endothelin 1 antibody [TR.ET.48.5]. Cells were probed without (left) or with(right) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute Dilution : 1:200 incubated overnight at 4 oC](https://www.genetex.com/upload/website/prouct_img/normal/GTX22786/GTX22786_439_ICC-IF_w_23060620_941.webp "ICC/IF analysis of Hela cells using GTX22786 Endothelin 1 antibody [TR.ET.48.5]. Cells were probed without (left) or with(right) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute Dilution : 1:200 incubated overnight at 4 oC")
![ICC/IF analysis of HUVEC cells using GTX22786 Endothelin 1 antibody [TR.ET.48.5]. Cells were probed without (left) or with(right) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute Dilution : 1:200 incubated overnight at 4 oC](https://www.genetex.com/upload/website/prouct_img/normal/GTX22786/GTX22786_440_ICC-IF_w_23060620_468.webp "ICC/IF analysis of HUVEC cells using GTX22786 Endothelin 1 antibody [TR.ET.48.5]. Cells were probed without (left) or with(right) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute Dilution : 1:200 incubated overnight at 4 oC")
![ICC/IF analysis of PC12 cells using GTX22786 Endothelin 1 antibody [TR.ET.48.5]. Cells were probed without (left) or with(right) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute Dilution : 1:200 incubated overnight at 4 oC](https://www.genetex.com/upload/website/prouct_img/normal/GTX22786/GTX22786_441_ICC-IF_w_23060620_369.webp "ICC/IF analysis of PC12 cells using GTX22786 Endothelin 1 antibody [TR.ET.48.5]. Cells were probed without (left) or with(right) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute Dilution : 1:200 incubated overnight at 4 oC")
FACS analysis of 293T cells using GTX22786 Endothelin 1 antibody [TR.ET.48.5] compared to an isotype control (blue). Dilution : 0.5 ug/test for 60 min at room temperature Fixation : 2% paraformaldehyde
Endothelin 1 antibody [TR.ET.48.5]
GTX22786
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin, RadioImmunoAssay
Product group Antibodies
ReactivityCanine, Human, Mouse, Porcine, Rat, Sheep
TargetEDN1
Overview
- SupplierGeneTex
- Product NameEndothelin 1 antibody [TR.ET.48.5]
- Delivery Days Customer9
- Antibody SpecificityThis antibody shows little cross-reactivity to related peptides and no known cross-reactivity to non-related peptides. Studies suggest that this antibody binds to an epitope in the region of ET-1 represented by amino acids 8-16.
- Application Supplier NoteWB: 1:100-1:1000. ICC/IF: 1:200-1:1000. IHC-P: 1:250. IHC-Fr: 1:250. FACS: 0.5 microg/test. RIA: 1:25,000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin, RadioImmunoAssay
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDTR.ET.48.5
- Concentration2.5 mg/ml
- ConjugateUnconjugated
- Gene ID1906
- Target nameEDN1
- Target descriptionendothelin 1
- Target synonymsARCND3; endothelin-1; ET1; HDLCQ7; PPET1; preproendothelin-1; QME
- HostMouse
- IsotypeIgG1
- Protein IDP05305
- Protein NameEndothelin-1
- Scientific DescriptionThis gene encodes a preproprotein that is proteolytically processed to generate a secreted peptide that belongs to the endothelin/sarafotoxin family. This peptide is a potent vasoconstrictor and its cognate receptors are therapeutic targets in the treatment of pulmonary arterial hypertension. Aberrant expression of this gene may promote tumorigenesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2015]
- ReactivityCanine, Human, Mouse, Porcine, Rat, Sheep
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Increased tissue endothelin-1 and endothelin-B receptor expression in temporal arteries from patients with giant cell arteritis. Dimitrijevic I et al., 2010 Mar, OphthalmologyRead more