Bio-Connect

ENO3 antibody

GTX103808
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
TargetENO3
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request

Overview

  • Supplier
    GeneTex
  • Product Name
    ENO3 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:5000-1:20000. ICC/IF: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID2027
  • Target name
    ENO3
  • Target description
    enolase 3
  • Target synonyms
    2-phospho-D-glycerate hydrolyase; beta-enolase; enolase 3 (beta, muscle); GSD13; MSE; muscle enriched enolase; muscle-specific enolase; skeletal muscle enolase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP13929
  • Protein Name
    Beta-enolase
  • Scientific Description
    This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in skeletal muscle cells in the adult. A switch from alpha enolase to beta enolase occurs in muscle tissue during development in rodents. Mutations in this gene can be associated with metabolic myopathies that may result from decreased stability of the enzyme. Two transcripts have been identified for this gene that differ only in their 5 UTR. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

Related products

Product group Antibodies
Assay Genie
TargetENO3
  • SizePrice
Product group Antibodies
Biomatik
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetENO3
  • SizePrice
Product group Antibodies
Antibodies.com
ApplicationsDot Blot, ImmunoFluorescence, Western Blot, ELISA
  • SizePrice
Product group Antibodies
References
GeneTex
ENO3 antibodyGTX113429
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetENO3
  • SizePrice
Product group Antibodies
MedChem Express
TargetENO3
  • SizePrice
Immunohistochemistry analysis in human skeletal muscle and tonsil tissues using Anti-ENO3 antibody. Corresponding ENO3 RNA-seq data are presented for the same tissues.
Product group Antibodies
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
TargetENO3
  • SizePrice
Immunohistochemistry of paraffin-embedded human colon cancer using CSB-CSB-PA13169A0Rb at dilution of 1:100
Product group Antibodies
Cusabio
ENO3 AntibodyCSB-PA13169A0RB
ApplicationsImmunoFluorescence, ELISA, ImmunoHistoChemistry
TargetENO3
  • SizePrice
Product group Antibodies
Biosynth
  • SizePrice
Product group Antibodies
RayBiotech
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
TargetENO3
  • SizePrice
Figure 1. Western blot analysis of ENO3 using anti-ENO3 antibody (A06845-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human U937 whole cell lysates, Lane 4: human Caco-2 whole cell lysates, Lane 5: rat skeletal muscle tissue lysates, Lane 6: rat heart tissue lysates, Lane 7: mouse skeletal muscle tissue lysates, Lane 8: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ENO3 antigen affinity purified polyclonal antibody (Catalog # A06845-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ENO3 at approximately 47 kDa. The expected band size for ENO3 is at 47 kDa.
Product group Antibodies
Boster Bio
Anti-ENO3 Antibody Picoband(r)A06845-3-CARRIER-FREE
ApplicationsFlow Cytometry, Western Blot, ELISA
TargetENO3
  • SizePrice