WB analysis of Bend3 cells using GTX42866 eNOS (phospho Ser1177) antibody [BDI250] at 1 μg/mL.
Lane 1 : Untreated whole cell lysate
Lane 2 : Pervanadate-treated whole cell lysate
WB analysis of Bend3 cells using GTX42866 eNOS (phospho Ser1177) antibody [BDI250] at 1 μg/mL.
Lane 1 : Untreated whole cell lysate
Lane 2 : Pervanadate-treated whole cell lysate
eNOS (phospho Ser1177) antibody [BDI250]
GTX42866
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse
TargetNOS3
Overview
- SupplierGeneTex
- Product NameeNOS (phospho Ser1177) antibody [BDI250]
- Delivery Days Customer9
- Application Supplier NoteWB: 2 microg/ml. ELISA: 0.1 microg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot, ELISA
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDBDI250
- Concentration0.10 mg/ml
- ConjugateUnconjugated
- Gene ID4846
- Target nameNOS3
- Target descriptionnitric oxide synthase 3
- Target synonymsECNOS, eNOS, nitric oxide synthase 3, EC-NOS, NOS type III, NOSIII, cNOS, constitutive NOS, endothelial NOS, nitric oxide synthase 3 (endothelial cell)
- HostMouse
- IsotypeIgG1
- Protein IDP29474
- Protein NameNitric oxide synthase 3
- Scientific DescriptionNitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Alternative splicing and the use of alternative promoters results in multiple transcript variants. [provided by RefSeq, Oct 2016]
- ReactivityHuman, Mouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
Datasheet
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![Untreated (–) and treated (+) HUVEC whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membranes were blotted with eNOS (phospho Ser1177) antibody [HL3498] (GTX641386) diluted at 1:500 and eNOS antibody (GTX133404) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX641386/GTX641386_T-45607_20250314_WB_treatment_Pervanadate_25031900_574.webp)
Product group Antibodies
ApplicationsWestern Blot
ReactivityHuman
TargetNOS3
- SizePrice
Product group Antibodies
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityMouse, Porcine
TargetNOS3
- SizePrice