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Western Blot analysis of HepG2 cells using EOMES Polyclonal Antibody
Western Blot analysis of HepG2 cells using EOMES Polyclonal Antibody
Western Blot analysis of HepG2 cells using EOMES Polyclonal Antibody

EOMES Antibody

CSB-PA002351
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetEOMES
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Overview

  • Supplier
    Cusabio
  • Product Name
    EOMES Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8320
  • Target name
    EOMES
  • Target description
    eomesodermin
  • Target synonyms
    eomesodermin homolog; T-box brain protein 2; TBR2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO95936
  • Protein Name
    Eomesodermin homolog
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TBR2/Eomes using anti-TBR2/Eomes antibody (A00992-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat spleen tissue lysates, Lane 2: rat thymus tissue lysates, Lane 3: mouse spleen tissue lysates, Lane 4: mouse thymus tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TBR2/Eomes antigen affinity purified polyclonal antibody (Catalog # A00992-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TBR2/Eomes at approximately 85KD. The expected band size for TBR2/Eomes is at 73KD.
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