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ProductsBack/Eotaxin antibody [7H5]

Eotaxin antibody [7H5]

GTX53259
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, Neutralisation/Blocking
Product group Antibodies
ReactivityMouse
TargetCcl11
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Overview

  • Supplier
    GeneTex
  • Product Name
    Eotaxin antibody [7H5]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500 - 1:1000. IHC-Fr: 1:100 - 1:300. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, Neutralisation/Blocking
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    7H5
  • Concentration
    500 ug/ml
  • Conjugate
    Unconjugated
  • Gene ID20292
  • Target name
    Ccl11
  • Target description
    C-C motif chemokine ligand 11
  • Target synonyms
    Scya11, eotaxin, eotaxin, C-C motif chemokine 11, chemokine (C-C motif) ligand 11, eosinophil chemotactic protein, small chemokine (C-C motif) ligand 11, small-inducible cytokine A11
  • Host
    Rat
  • Isotype
    IgG2
  • Protein IDP48298
  • Protein Name
    Eotaxin
  • Reactivity
    Mouse
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Eotaxin using anti-Eotaxin antibody (PB9065). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse lung tissue lysates, Lane 2: mouse intestines tissue lysates, Lane 3: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Eotaxin antigen affinity purified polyclonal antibody (Catalog # PB9065) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Eotaxin at approximately 11 kDa. The expected band size for Eotaxin is at 11 kDa.
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