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Western blot All lanes: EPHX1 antibody at 2microg/ml + Hela whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 53 kDa Observed band size: 53 kDa
Western blot All lanes: EPHX1 antibody at 2microg/ml + Hela whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 53 kDa Observed band size: 53 kDa
Western blot All lanes: EPHX1 antibody at 2microg/ml + Hela whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 53 kDa Observed band size: 53 kDa

EPHX1 Antibody

CSB-PA007734LA01HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetEPHX1
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Overview

  • Supplier
    Cusabio
  • Product Name
    EPHX1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2052
  • Target name
    EPHX1
  • Target description
    epoxide hydrolase 1
  • Target synonyms
    EPHX, EPOX, HYL1, MEH, epoxide hydrolase 1, epoxide hydratase, epoxide hydrolase 1 microsomal, epoxide hydrolase 1, microsomal (xenobiotic)
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP07099
  • Protein Name
    Epoxide hydrolase 1
  • Scientific Description
    Biotransformation enzyme that catalyzes the hydrolysis of arene and aliphatic epoxides to less reactive and more water soluble dihydrodiols by the trans addition of water.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of Epoxide hydrolase/EPHX1 using anti-Epoxide hydrolase/EPHX1 antibody (A00899-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human HCCT tissue lysates, Lane 3: human HCCP tissue lysates, Lane 4: rat liver tissue lysates, Lane 5: rat RH35 whole cell lysates, Lane 6: mouse liver tissue lysates, Lane 7: mouse HEPA1-6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Epoxide hydrolase/EPHX1 antigen affinity purified polyclonal antibody (Catalog # A00899-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Epoxide hydrolase/EPHX1 at approximately 53 kDa. The expected band size for Epoxide hydrolase/EPHX1 is at 53 kDa.
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