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ProductsBack/EPHX2 Mouse Monoclonal Antibody [Clone ID: OTI3F6]
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EPHX2 (RC202489, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EPHX2. Positive lysates LY419612 (100ug) and LC419612 (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EPHX2 (RC202489, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EPHX2. Positive lysates LY419612 (100ug) and LC419612 (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EPHX2 (RC202489, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EPHX2. Positive lysates LY419612 (100ug) and LC419612 (20ug) can be purchased separately from OriGene.

EPHX2 Mouse Monoclonal Antibody [Clone ID: OTI3F6]

CF501628
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot
Product group Antibodies
ReactivityHuman
TargetEPHX2
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    EPHX2 Mouse Monoclonal Antibody [Clone ID: OTI3F6]
  • Delivery Days Customer
    14
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI3F6
  • Gene ID2053
  • Target name
    EPHX2
  • Target description
    epoxide hydrolase 2
  • Target synonyms
    ABHD20, CEH, SEH, bifunctional epoxide hydrolase 2, epoxide hydratase, epoxide hydrolase 2, cytoplasmic, epoxide hydrolase 2, cytosolic, epoxide hydrolase, soluble
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP34913
  • Protein Name
    Bifunctional epoxide hydrolase 2
  • Scientific Description
    Carrier-free (BSA/glycerol-free) EPXH2 mouse monoclonal antibody, clone OTI3F6 (formerly 3F6)
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of EPHX2 using anti-EPHX2 antibody (A01999-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: rat liver tissue lysates, Lane 3: rat kidney tissue lysates, Lane 4: mouse liver tissue lysates, Lane 5: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EPHX2 antigen affinity purified polyclonal antibody (Catalog # A01999-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EPHX2 at approximately 63 kDa. The expected band size for EPHX2 is at 63 kDa.
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