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IHC-P analysis of human colon tissue using GTX31940 EPLIN antibody. Working concentration : 5 microg/ml
IHC-P analysis of human colon tissue using GTX31940 EPLIN antibody. Working concentration : 5 microg/ml
IHC-P analysis of human colon tissue using GTX31940 EPLIN antibody. Working concentration : 5 microg/ml

EPLIN antibody

GTX31940
GeneTex
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetLIMA1
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Overview

  • Supplier
    GeneTex
  • Product Name
    EPLIN antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 0.5 - 1 microg/mL. IHC-P: 5 microg/mL. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID51474
  • Target name
    LIMA1
  • Target description
    LIM domain and actin binding 1
  • Target synonyms
    EPLIN, LDLCQ8, SREBP3, LIM domain and actin-binding protein 1, epithelial protein lost in neoplasm beta, sterol regulatory element binding protein 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UHB6
  • Protein Name
    LIM domain and actin-binding protein 1
  • Scientific Description
    This gene encodes a cytoskeleton-associated protein that inhibits actin filament depolymerization and cross-links filaments in bundles. It is downregulated in some cancer cell lines. Alternatively spliced transcript variants encoding different isoforms have been described for this gene, and expression of some of the variants maybe independently regulated. [provided by RefSeq, Aug 2011]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of EPLIN/LIMA1 using anti-EPLIN/LIMA1 antibody (A05231-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human T-47D whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EPLIN/LIMA1 antigen affinity purified polyclonal antibody (Catalog # A05231-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EPLIN/LIMA1 at approximately 85 kDa and 100 kDa. The expected band size for EPLIN/LIMA1 is at 85 kDa.
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