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Epoxide Hydrolase 1 Antibody (YA2895)

HY-P83150
MedChem Express
TargetEPHX1
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    Epoxide Hydrolase 1 Antibody (YA2895)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID2052
  • Target name
    EPHX1
  • Target description
    epoxide hydrolase 1
  • Target synonyms
    EPHX; EPOX; epoxide hydratase; epoxide hydrolase 1; epoxide hydrolase 1 microsomal; epoxide hydrolase 1, microsomal (xenobiotic); HYL1; MEH
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    Epoxide Hydrolase 1 Antibody (YA2895) is a non-conjugated IgG antibody, targeting Epoxide Hydrolase 1, with a predicted molecular weight of 63 kDa (observed band size: 63 kDa). Epoxide Hydrolase 1 Antibody (YA2895) can be used for WB experiment in human, mouse background.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Epoxide hydrolase/EPHX1 using anti-Epoxide hydrolase/EPHX1 antibody (A00899-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human HCCT tissue lysates, Lane 3: human HCCP tissue lysates, Lane 4: rat liver tissue lysates, Lane 5: rat RH35 whole cell lysates, Lane 6: mouse liver tissue lysates, Lane 7: mouse HEPA1-6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Epoxide hydrolase/EPHX1 antigen affinity purified polyclonal antibody (Catalog # A00899-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Epoxide hydrolase/EPHX1 at approximately 53 kDa. The expected band size for Epoxide hydrolase/EPHX1 is at 53 kDa.
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