Sample (30 ug of whole cell lysate) A: H1299 B: Hela 7.5% SDS PAGE EPS8 antibody GTX100254 diluted at 1:1000
![EPS8 antibody [C3], C-term detects EPS8 protein at cytosol and nucleus on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. EPS8 antibody [C3], C-term (GTX100254) dilution: 1:500.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min](https://www.genetex.com/upload/website/prouct_img/normal/GTX100254/GTX100254_41724_IHC_w_23060100_129.webp "EPS8 antibody [C3], C-term detects EPS8 protein at cytosol and nucleus on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. EPS8 antibody [C3], C-term (GTX100254) dilution: 1:500.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min")
![Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EPS8 antibody [C3], C-term (GTX100254) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX100254/GTX100254_39568_20170518_WB_M_R_w_23060100_434.webp "Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EPS8 antibody [C3], C-term (GTX100254) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Immunoprecipitation of EPS8 protein from HepG2 whole cell extracts using 5 μg of EPS8 antibody [C3], C-term (GTX100254). Western blot analysis was performed using EPS8 antibody [C3], C-term (GTX100254). EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.](https://www.genetex.com/upload/website/prouct_img/normal/GTX100254/GTX100254_39568_20150529_IP_w_23060100_246.webp "Immunoprecipitation of EPS8 protein from HepG2 whole cell extracts using 5 μg of EPS8 antibody [C3], C-term (GTX100254). Western blot analysis was performed using EPS8 antibody [C3], C-term (GTX100254). EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.")
Sample (30 ug of whole cell lysate) A: H1299 B: Hela 7.5% SDS PAGE EPS8 antibody GTX100254 diluted at 1:1000
EPS8 antibody [C3], C-term
GTX100254
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetEPS8
Overview
- SupplierGeneTex
- Product NameEPS8 antibody [C3], C-term
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID2059
- Target nameEPS8
- Target descriptionEGFR pathway substrate 8, signaling adaptor
- Target synonymsDFNB102, epidermal growth factor receptor kinase substrate 8, epidermal growth factor receptor pathway substrate 8
- HostRabbit
- IsotypeIgG
- Protein IDQ12929
- Protein NameEpidermal growth factor receptor kinase substrate 8
- Scientific DescriptionThis gene encodes a member of the EPS8 family. This protein contains one PH domain and one SH3 domain. It functions as part of the EGFR pathway, though its exact role has not been determined. Highly similar proteins in other organisms are involved in the transduction of signals from Ras to Rac and growth factor-mediated actin remodeling. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized. [provided by RefSeq]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
Datasheet
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